Generator

Part:BBa_K1368007

Designed by: Yihui Zheng   Group: iGEM14_HZAU-China   (2014-09-30)

pluxR+taRNA+Ter

The right luxr promoter gives relatively weak constitutive expression of downstream genes. LuxR in concert with HSL binds to a palindromic site on the right luxr promoter, increasing the rate of transcription. taRNA is taR12 which is bioBricked version of Isaacs' riboregulator trans-activating key. After the right luxr promoter being activated by the complex of LuxR in concert with HSL, the increasing taR12 key will activate the corresponding crR12 lock. The hybrid design allows for the tight control of the expression of downstream gene driven by HSL

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61


[edit]
Categories
Parameters
n/apluxR+taRNA+Ter